Oct4 Antibody from Novus Biologicals

Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

Oct4 Antibody

Catalog Number:

NB100-2379

Clone Number:

My research is focused on the response of human cancer cells, specifically breast cancer cells, to different drugs, and the molecular mechanisms underlying their response. This antibody was used to detect Oct4 expression in response to beta-mercaptoethanol or glutathione treatment in combination with digoxin in SUM-149 cells by western blot analysis.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human breast cancer cell line

Primary Incubation

1:2000

Blocking Agent

5% milk

Secondary Incubation

1:3000

Tertiary Incubation

N/A

Detection

Enhanced chemiluminescence detection kit

Results Summary

This antibody detected Oct4 expression in SUM-149 cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Signal is strong and specific.

The Bad

N/A

The Bottom Line

This is a perfect antibody to detect Oct4 expression by western blot.

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