KLF4 Antibody from Novus Biologicals

Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

KLF4 Antibody

Catalog Number:

NBP1-83940

My research is focused on the response of human cancer cells, specifically breast cancer cells, to different drugs, and the molecular mechanisms underlying their response. This antibody was used to detect KLF4 expression in response to carboplatin treatment in human breast cancer cells by western blot analysis.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human breast cancer cell line

Primary Incubation

1:1000

Blocking Agent

5% milk

Secondary Incubation

1:2000

Tertiary Incubation

N/A

Detection

Enhanced chemiluminescence detection kit

Results Summary

This antibody detected KLF4 expression in human breast cancer cells, but with several non-specific bands.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Can detect KLF4 band.

The Bad

Cannot be recycled for many times. Better to blot with fresh antibody.

The Bottom Line

This antibody can be used to detect KLF4 expression by western blot in human breast cancer cells.

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