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Alexa Fluor® 488 anti-mouse CD3 Antibody
100210
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We study the role of lymphocytes in epithelial tumorigenesis in mammary gland. We chose this a-CD3 antibody due to its reliability.
Immunofluorescence
PLP fixed mouse (C57BL/6) virgin mammary gland cryosections
1 in 200
1% BSA, 1% FCS, 0.3% Triton X, 2% mouse serum, 2% rat serum
None
Images were acquired using an inverted Zeiss LSM880 confocal microscope with a 20x 0.8NA air objective.
CD3 is in red, E-cadherin is in white and Hoechst in blue. On the left, CD3+ cells in the lymph node can be seen.
N/A
Reliable & reproducible staining of lymphocytes
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