Good CD49a Antibody

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

cd49a PECy7 anti-human

Catalog Number:

328312

Clone Number:

One of our lab studies focuses on charactering different T cell types throughout the human body. We chose this antibody because it’s bright enough for us to see these cells, but not too bright that any subtle expression changes are masked.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

human lung cells

Primary Incubation

Cells were stained in 100 uL FACS buffer with antibody at 1:100; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100 uL FACS buffer.

Blocking Agent

FACS

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II

Results Summary

This antibody allows us to see CD49a expressing cells in our samples.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright and stable

The Bad

None

The Bottom Line

This antibody works efficiently to stain human CD49a expression.

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