Works Great and Easy Optimization

Pharmacology
Duke University
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Cas9 Antibody

Catalog Number:

19526

Our lab is interested in studying the role of inflammatory kinases as a therapeutic target. We perform Elisa and western blot assays to determine the role and pro inflammatory status of macrophages in response to pharmacological Inhibition of these kinases. This antibody works every time and will detect even at low dilutions which makes a little antibody go a long way!

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Macrophages

Primary Incubation

1:2,000

Blocking Agent

Normal Donley Serum

Secondary Incubation

1:2,000 Ax 648 HRP

Tertiary Incubation

None

Detection

Western blot chemiluminescence

Results Summary

This works every time and has a good detection. Just make sure the protein has transferred to the membrane before probing. This antibody works for most blocking and most tissue samples I have ever used with it.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works great

The Bad

None noted

The Bottom Line

Works well and is easy to use for results.

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