Great and Easy for Detection

Pharmacology
Duke University
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Ly6G anti mouse Ax488

Catalog Number:

127626

Clone Number:

Our lab is interested in studying the role of inflammatory kinases as a therapeutic target. We perform Elisa and western blot assays to determine the role and pro inflammatory status of macrophages in response to pharmacological Inhibition of these kinases. This antibody worked great to identify neutrophils in blood samples from mice. The stain was easy and created consistent results with what we expected to see.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse blood

Primary Incubation

1:100

Blocking Agent

CD16/32

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSRC II

Results Summary

Works great and has a good fluorescence in the samples. This is a good antibody to detect neutrophils.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to use.

The Bad

None noted

The Bottom Line

This is a fast and easy antibody which provides a good level of detection.

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