Ok for Dendritic Cell Detection

Pharmacology
Duke University
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Cd11c PE

Catalog Number:

117307

Clone Number:

Our lab is interested in studying the role of inflammatory kinases as a therapeutic target. We perform Elisa and western blot assays to determine the role and pro inflammatory status of macrophages in response to pharmacological Inhibition of these kinases. This product provides a semi weak signal for Cd11c, not the best signal so controls need to be tight for detecting positive cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Spleen

Primary Incubation

1:50

Blocking Agent

Cd16/32

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSRC II

Results Summary

This is not the best for low detection but may work to identify populations of cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

Not the best fluorescence.

The Bottom Line

This is an ok antibody for flow cytometery.

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