Fluorescent Phalloidin

March 08, 2019

BlueskyReddit
Physiology
Jali Medical Inc.
Research Scientist

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Company:

Thermo Fisher Scientific

Product Name:

Phalloidin-conjugated to Alexafluor 568

Catalog Number:

A12380

Browse Similar Products: F-Actin Immunofluorescence Antibodies

Fluorescent phalloidin was used to stain neuronal dendrites for spine density counts in pre-adolescent compared to adolescent mice. At the scale of spines the dilution required to visualize and do counts using confocal microscopy was 1:20. Scale bar of image is 10 micrometers.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse Brain

Primary Incubation

N/A

Blocking Agent

1% bovine serum albumin

Secondary Incubation

Blocked in 0.01 M PBS supplemented with 1% bovine serum albumin, 0.25% Triton and 0.05% sodium azide for 24 hr

Tertiary Incubation

Ab in in 0.01 M PBS for 24 hours

Detection

Fluorescence

Results Summary

To detect and do neuronal spine density counts, a dilution of 1:20 was required of this antibody. Additionally, the resolution of images was highly dependent on the quality of the perfusion and only with overlapping stains was there accurate consensus of whether the stain was staining the appropriate structures in certain cases. Scale bar of images is 2.5 micrometers.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Ab conjugated with fluorescence

The Bad

Not great resolution

The Bottom Line

The idea of an antibody conjugated with fluorescence is appealing; however, this antibody required a very high concentration to produce usable stained mouse brain slices.

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