Cheap DNA Purification Mini-Prep Column

Harvard Medical School
Cell Biology
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thomas Scientific

Product Name:

SpinSmart™ Plasmid Miniprep DNA Purification Columns

Catalog Number:

1158P44

Very often, we run into the situation when the columns in the mini-prep or PCR cleanup kit run out, but there is still a lot of buffer left. Instead of buying the whole kit, we buy the columns and use up the rest of buffer. This SpinSmart column is a choice. In my hands, they have worked totally fine just like the columns in the original kit for mini-prep, PCR cleanup, and gel purification. It saves money.

Experimental Design and Results Summary

Application

DNA purification

Starting Material

N/A

Protocol Overview

Just apply the solution, spin down, wash with wash buffer, and then elute with water or elution buffer.

Tips

Work with it in the same way as the column in kit.

Results Summary

Works as well as the column from original kit.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Cheap and reliable

The Bad

Some people are worried that it cannot clean up toxin as well as columns from the kit

The Bottom Line

These columns work very well if you are doing mini-prep, PCR cleanup, or gel purification for cloning.

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