Anti-Vinculin Antibody

Biology and Biochemistry
University of Houston
Graduate student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Sigma-Aldrich

Product Name:

Anti-Vinculin Antibody

Catalog Number:

V4505

Browse Similar Products: Vinculin Western Blot Antibodies

Currently, there are no targeted therapies for triple negative breast cancer patients. Our lab focused on identifying novel molecules to develop targeted therapies for triple negative breast cancer patients. Our research program is involved in characterizing protein kinases and transcription factors as therapeutic targets for these patients. We used this antibody in western blot to detect vinculin protein.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Protein sample from human cancer cells

Primary Incubation

Antibody was diluted at 1:8000 in 5% milk for overnight at 4°C on shaker

Blocking Agent

5% milk in 0.01% TBS-T

Secondary Incubation

Anti-mouse IgG conjugated with HRP diluted at 1:8000 for 1 hour at room temperature on shaker

Tertiary Incubation

N/A

Detection

ECL and Film

Results Summary

This antibody produces clean bands for vinculin protein.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very good antibody

The Bad

Nothing

The Bottom Line

Highly recommend this antibody as loading control for western blot analysis.

Share your experience with other scientists. Write a Review! »

Join the discussion