Anti-FOXM1 Antibody

Biology and Biochemistry
University of Houston
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

FOXM1 antibody

Catalog Number:

sc-376620

Currently, there are no targeted therapies for triple negative breast cancer patients. Our lab focused on identifying novel molecules to develop targeted therapies for triple negative breast cancer patients. Our research program is involved in characterizing protein kinases and transcription factors as therapeutic targets for these patients. We used this antibody to detect FOXM1 protein.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Protein sample from human cancer cells

Primary Incubation

Antibody was diluted at 1:3000 in 5% milk for overnight at 4°C on shaker

Blocking Agent

5% milk in 0.01% TBS-T

Secondary Incubation

Anti-mouse IgG conjugated with HRP diluted at 1:4000 for 1 hour at room temperature on shaker

Tertiary Incubation

N/A

Detection

ECL and Film

Results Summary

Very good FOXM1 antibody and easily detects FOXM1 proteins (including all of its isoforms ).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Very good antibody

The Bad

Nothing

The Bottom Line

I recommend this antibody for use in western blotting to detect FOXM1.

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