Anti-V5-Tag Antibody

Biology and Biochemistry
University of Houston
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signalling Technology

Product Name:

V5-tag antibody

Catalog Number:

13202

Currently, there are no targeted therapies for triple negative breast cancer patients. Our lab focused on identifying novel molecules to develop targeted therapies for triple negative breast cancer patients. Our research program is involved in characterizing protein kinases and transcription factors as therapeutic targets for these patients. We used this antibody to detect V5-tagged GFP protein.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Protein sample from human cancer cells

Primary Incubation

Antibody was diluted at 1:5000 in 5% milk for overnight at 4°C on shaker

Blocking Agent

5% milk in 0.01% TBS-T

Secondary Incubation

Anti-rabbit IgG conjugated with HRP

Tertiary Incubation

N/A

Detection

ECL and Film

Results Summary

This antibody was used to detect V5-tagged proteins. This antibody works really for western blot analysis and produces clear band.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very good antibody

The Bad

Nothing

The Bottom Line

Very good antibody

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