Anti-PTK7 Antibody

Biology and Biochemistry
University of Houston
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

PTK7/CCK4 antibody

Catalog Number:

MAB4499

Currently, there are no targeted therapies for triple negative breast cancer patients. Our lab focused on identifying novel molecules to develop targeted therapies for triple negative breast cancer patients. Our research program is involved in characterizing protein kinases and transcription factors as therapeutic targets for these patients. We used this antibody in western blot to detect human PTK7 protein.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Protein sample from human cancer cells

Primary Incubation

Antibody was diluted at 1:1000 in 5% milk for overnight at 4°C on shaker

Blocking Agent

5% milk in 0.01% TBS-T

Secondary Incubation

Anti-mouse IgG conjugated with HRP

Tertiary Incubation

N/A

Detection

ECL and Film

Results Summary

This antibody produces a band for PTK7 protein western blot analysis and confirmed with shRNA.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good antibody

The Bad

Need to use strong ECL reagent to detect PTK7 protein with this antibody

The Bottom Line

Good antibody

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