Anti-MELK Antibody

Biology and Biochemistry
University of Houston
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

MELK antibody

Catalog Number:

AF4820

Currently, there are no targeted therapies for triple negative breast cancer patients. Our lab focused on identifying novel molecules to develop targeted therapies for triple negative breast cancer patients. Our research program is involved in characterizing protein kinases and transcription factors as therapeutic targets for these patients. We used this antibody in western blot analysis to detect MELK protein.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Protein sample from human cancer cells

Primary Incubation

This antibody was used in western blot analysis to detect MELK protein.

Blocking Agent

5% milk in 0.01% TBS-T

Secondary Incubation

Anti-sheep IgG conjugated with HRP

Tertiary Incubation

N/A

Detection

ECL and Film

Results Summary

This antibody was used to detect MELK protein through western blotting analysis. This antibody works very well and produces a band around molecular weight of 75 kDa. This band disappeared when the cells were treated with siRNA against MELK.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Very good antibody

The Bad

Sometimes it gives two bands

The Bottom Line

Works very well. Best antibody to detect MELK.

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