R&D systems
Recombinant Mouse E-Selectin/CD62E Fc Chimera Protein, CF
575-ES-100
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This was used to detect basal and modified levels of E-selectin interaction for a variety of tumor cells. This product is highly sensitive compared to the human construct.
Flow Cytometry
Human cancer cells
1:100
10% FBS
Al647 anti-human
none
BD LSR2
This fusion product consistently detected a binding interaction and was robust to multiple protocol variations. Must be used with Ca2+ and Mg2_ supplemented binding medium as well. Binding is stronger for this mouse construct than the human E-selectin.
N/A
Highly robust, specific
Pricey
This is the standard reagent for E-selectin binding experiments and papers