Thermo Fisher Scientific
F4/80 Monoclonal Antibody (BM8), eFluor 660
50-4801-82
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We are interested in the role of kidney resident macrophages in kidney inflammation. We wanted to investigate the location of kidney macrophages in relation to endothelial cells and were looking for a good antibody to stain macrophages.
Immunofluorescence
PLP fixed mouse (C57BL/6) kidney cryosections
eFluor 660 anti-mouse F4/80 (5 µg/mL) for 3 hr at room temperature
1% BSA, 1% FCS, 0.3% Triton X, 2% mouse serum, 2% rat serum
N/A
Images were acquired using an inverted Zeiss LSM880 confocal microscope with a 20x 0.8NA air objective.
Excellent staining of macrophages (green). Endothelial cells are in red.
27477514
Excellent staining
None
Excellent staining of macrophages, using directly conjugated antibody.