Tocris Bioscience
Akti-1 / 2
5773/10
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LPA treatment induced Akt phosphorylation in osteosarcoma cells. Therefore, we used Akt-1/2 to investigate role of Akt in COX-2 activation.
Cell culture
Human osteosarcoma cells
Human osteosarcoma cells were incubated with 10 μM Akti-1/2 for 30 min prior to treatment with 10 μM LPA to estimate COX-2 expression using Western blot analysis.
N/A
Akti-1/2 abolished LPA-induced COX-2 expression.
Worked as expected
Akt-1/2 is a selective and effective Akt inhibitor.