Non-Specific Nuclear Staining in the Mouse Brain with TMEM119

February 13, 2019

Neuroscience
Wisconsin University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

Purified anti-TMEM119 (Extracellular) Antibody

Catalog Number:

853301

We are seeking to understand microglial activation in the mouse brain. This antibody is expected to stain microglia as TMEM119 is known to be a microglia specific marker. However, the result showed unspecific nuclear staining in multiple cell types in contrast to microglial specific cytoplasmic staining. To be fair, BioLegend advertises this antibody for use in human tissue, while we were using it in mouse tissue.

View Comments

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse Brain (manufacturer has not tested product in mouse)

Primary Incubation

1:100 for overnight at 4°C

Blocking Agent

1% BSA, 0.2% Triton X, 10% donkey serum

Secondary Incubation

1:500 dilution 1 hr at 25°C in blocking reagent

Tertiary Incubation

N/A

Detection

N/A

Results Summary

Cryosections of murine brain tissue were stained for TMEM119 (Green) and counterstained with DAPI (blue).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Sample size available

The Bad

Non-specific for mouse microglia

The Bottom Line

Does not work for murine microglia but this reactivity was not confirmed with other species. Manufacturer claims this antibody should work in human tissue.

Share your experience with other scientists. Write a Review! »

Join the discussion