BioLegend
Anti-mouse H-2Db APC
111513
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This antibody was used to stain the H-2Db MHC class I alloantigen in splenocytes isolated from C57Bl/6 mice. It was chosen because it is a commonly used clone for H-2Db, APC is bright and works well with other fluorophores in the staining panel, and BioLegend had a competitive price.
Flow Cytometry
mouse splenocytes
1:100 dilution in 100 µL total staining volume for 15 minutes at room temperature.
FcBlock + 2% FBS
N/A
BD LSR II
H-2Db staining was excellent (filled histogram) as compared to unstained cells (dotted line).
Works well. Reproducible results. Low price.
Excellent staining and reproducible results. Will continue to use this antibody.