BioLegend
Anti-mouse CD69 APC
104513
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This antibody was used as a T cell activation marker in mouse splenocytes stimulated with PMA/ionomycin. It was chosen because it is a commonly used clone for CD69, APC is bright and works well with other fluorophores in the staining panel, and BioLegend had a competitive price.
Flow Cytometry
Mouse splenocytes
1:100 dilution in 100 µL total staining volume for 15 minutes at room temperature.
FcBlock + 2% FBS
N/A
BD LSR II
CD69 staining was excellent (filled histogram) as compared to unstained cells (dotted line).
Works well. Reproducible results. Low price.
Excellent staining and reproducible results. Will continue to use this antibody.