Staining Mouse Splenocytes with Anti-CD44 APC

March 19, 2019

BlueskyReddit
Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

Anti-mouse CD44 APC

Catalog Number:

103011

This antibody was used to stain CD44+ cells in mouse splenocytes. It was chosen because it is a commonly used clone for CD44, APC is bright and works well with other fluorophores in the staining panel, and BioLegend had a competitive price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:100 dilution in 100 µL total staining volume for 15 minutes at room temperature.

Blocking Agent

FcBlock + 2% FBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSR II

Results Summary

CD44 staining was excellent (filled histogram) as compared to unstained cells (dotted line).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works well. Reproducible results. Low price.

The Bad

N/A

The Bottom Line

Excellent staining and reproducible results. Will continue to use this antibody.

Share your experience with other scientists. Write a Review! »

Join the discussion