BioLegend
Anti-mouse CD4 APC
100411
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This antibody was used as a marker for CD4+ T cells in mouse splenocytes. It was chosen because it is a commonly used clone for CD4, APC is bright and works well with other fluorophores in the staining panel, and BioLegend had a competitive price.
Flow Cytometry
Mouse splenocytes
1:100 dilution in 100 µL total staining volume for 15 minutes at room temperature.
FcBlock + 2% FBS
N/A
BD LSR II
CD4 staining was excellent. Positive cells were resolved from negative.
Works well. Reproducible results. Low price.
Excellent staining and reproducible results. Will continue to use this antibody.