Staining Mouse Splenocytes with Rat IgG2a PE Isotype Control

March 07, 2019

BlueskyReddit
Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Rat IgG2a PE isotype control

Catalog Number:

400507

This antibody was used as an isotype control to account for non-specific background staining in mouse splenocytes. It was chosen because it was the recommended isotype control for an antibody in a phenotyping panel, and BioLegend had a competitive price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:100 dilution in 100 µL total staining volume for 15 minutes at room temperature.

Blocking Agent

FcBlock + 2% FBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSR II

Results Summary

Isotype staining was significant (filled histogram) as compared to unstained cells (dotted line).

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works well. Reproducible results. Low price.

The Bad

N/A

The Bottom Line

Will continue to use this isotype control to account for background staining.

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