BioLegend
Anti-mouse CD8 BV421
100753
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This antibody was used to stain CD8+ cells in mouse splenocytes. It was chosen because it is a commonly used clone for CD8, BV421 is bright and works well with other fluorophores in the staining panel, and BioLegend had a competitive price.
Flow Cytometry
Mouse splenocytes
1:100 dilution in 100 µL total staining volume for 15 minutes at room temperature.
FcBlock + 2% FBS
N/A
BD LSR II
CD8 staining was excellent. Positive cells were fully resolved from negative.
Works well. Reproducible results. Low price.
Excellent staining, reproducible results, and low price. I'll continue to use this antibody.