Staining Mouse Splenocytes with Anti-CD4 FITC

January 29, 2019

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Anti-mouse CD4 FITC

Catalog Number:

100510

This antibody was used to stain CD4+ cells in mouse splenocytes. It was chosen because it is a commonly used clone for CD4, FITC has the appropriate brightness index for use with CD4 and works well with other fluorophores in the staining panel, and BioLegend had a competitive price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:100 dilution in 100 µL total staining volume for 15 minutes at room temperature.

Blocking Agent

FcBlock + 2% FBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSR II

Results Summary

CD4 staining was excellent. Positive cells were fully resolved from negative.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

CD4 staining was excellent. Positive cells were fully resolved from negative.

The Bad

N/A

The Bottom Line

Excellent staining. I'll continue to use this antibody.

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