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CD39 Biotin
135704
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Browse Similar Products: CD39 Flow Cytometry Antibodies
This antibody was difficult to optimize for flow cytometry. Here, we attempted to stain blood and spleen macrophages for CD39 expression. We tried live and post fixed cells. Live cells gave low signal and high background, whereas fixed cells gave almost no signal.
Flow Cytometry
Spleen/Blood
1:100
CD16/32 5%BSA
BV570 Streptavidin
None
BD LSR ii
This antibody was difficult to optimize and gave varying results depending on the secondary used. We found that live cells only worked and PFA fixed cells gave minimal signal.
N/A
Can be used for more flexible fluorochrome panel
Poor detection
This antibody needs to be optimized and gives varying results depending on staining protocols.