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APC/Cy7 anti-mouse I-A/I-E Antibody
107628
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This antibody was great at detecting MHCii expression on macrophages isolated from blood and spleen of C57 and Balbc mice. Good fluorescence at low concentrations (1:1000).
Flow Cytometry
Mice Spleen/Blood
1:1000
CD16/32
None - tandem fluorochrome
None
BD LSR II
This antibody worked great to pick up MHCii expression on immune cells isolated from spleen and blood. There was minimal/expected compensation needed. This fluorochrome remained stable for up to 48 hours post staining and PFA fixation of cells.
N/A
Works great for flow cytometry.
None noted
This is a great fluorochrome antibody for quick consistent flow cytometry use.