Great CD10 Antibody

Toxicology
University of Kentucky
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

CD10 APC

Catalog Number:

340922

Browse Similar Products: CD10 APC Flow Cytometry Antibodies

CD10 is a useful marker in the classification of pre B ALL. It is positive in 89% of cases. Our work on the identification of novel targets for B-ALL treatment require the accurate classification of the cases prior to testing new therapies. Using this anti-CD10 antibody allows us part of that classification process. We use it because it gives a good, bright signal and does not require too much optimization. We use it with CD19 because pre B ALL cells characteristically co-express CD10 and CD19.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human white blood cells

Primary Incubation

15 minutes, 4°C in the dark

Blocking Agent

None needed

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer equipped with 633 red laser and 660/20 filter

Results Summary

The results shown in the figure are typical. CD10 expression on mononuclear cells is unmistakable. It shows a clear separation and a nice ~50:50 positivity. Even when plotted against CD19, the separation is good and allows for clear classification of pre B-ALL cases.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bright signal. Easy optimization.

The Bad

No bad points!

The Bottom Line

For pre B-ALL classification, this antibody works well. It gives a bright signal and requires minimal optimization. Recommended!

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