Anti-CD45 APC

March 01, 2019

Biology and Biochemistry
University of Houston
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-mouse CD45 Antibody

Catalog Number:

103111

This antibody was used in flow cytometry analysis to sort leukocytes from non-leukocytes and tumor cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes and solid tumors

Primary Incubation

Antibody was diluted at 1:200 in FACS staining buffer for 20 minutes on ice

Blocking Agent

Truestain FcX at 1:50 dilution in FACS staining buffer

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS

Results Summary

My research involves the profiling of immune cells in spleen and tumors. To profile immune cells from crude spleen and tumor samples, we stain these samples with anti-CD45 which is a common antigen expressed in all leukocytes. Using CD45-staining, we will sort leukocytes. This antibody works really well and produces great results with no difficulty in detection and optimization at high dilution.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Very good antibody and less expensive

The Bad

None

The Bottom Line

This is a great antibody that produces great results in separating leukocytes from non-leukocytes. I highly recommend this antibody for immune profiling studies and this antibody is much less expensive than others.

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