Roti-Block

Leibniz-Institute of Molecular Pharmacology
Proteostasis in Aging and Disease
Ph.D. Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Carl Roth

Product Name:

Roti-Block

Catalog Number:

A151

This solution is great for blocking membranes for dot/western blots.

Experimental Design and Results Summary

Application

Blocking membranes for dot/western blots

Starting Material

Protein containing membranes.

Protocol Overview

Dilute the solution 1/10. Then make a solution containing TBST and 10% of this diluted rotiblock. Block your membrane for 15 minutes before proceeding with the desired primary antibody.

Tips

Block for 30 minutes max.

Results Summary

Blocks as well as blocking with milk.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Blocks as well as milk

The Bad

More expensive than milk

The Bottom Line

Recommended if there is no milk for blocking your membranes

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