Thermo Fischer Scientific
Pierce BCA Protein Assay Kit
23225
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Easy to use and creates a very precise standard curve.
BCA assay for protein quantification
Protein
Make a solution of the reagents A and B at a ration of 50:1 and plate 200 µL in a clear 96-well plate. Create your standard curve with BSA and prepare your proteins. Add 10 µL of protein (from the ones you wish to measure or from the standard curve) to a well containing the BCA solution. Incubate at 37 degrees for 30 minutes. Read on a plate reader.
For this protocol, I use a standard curve from 0–2 mg/mL of protein
I always achieve a precise, replicable standard curve and replicable quantification measurements for my proteins.
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Easy to use and you get replicable results.
You have to use quite a bit of protein for this assay.
This is my go-to assay for protein quantification and I always use this kit for my BCA assay needs.