Efficient Staining of CD3+ Cells

Center for Infectious Medicine
Karolinska Institute
Postdoctoral Researcher

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Company:

BD Biosciences

Product Name:

BV786 Mouse Anti-Human CD3

Catalog Number:

565491

Browse Similar Products: CD3 Flow Cytometry Antibodies

PBMC of a healthy donor were stained with viability dye and anti-human CD3-BV786 (BD, clone UCHT1) at varying titers (0; 1:1,600; 1:800; 1:400; 1:200; 1:100; 1:50; 1:25; 1:12.5). The antibody gives a clear separation and bright staining.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMC

Primary Incubation

15 min at room temperature (at varying titers)

Blocking Agent

Included in staining buffer (PBS containing EDTA and 2% FCS)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry (BD LSR Fortessa)

Results Summary

Clear separation of positive and negative events even at low titers (1:200 or 1:100) after gating on single viable lymphocytes.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bright staining at low titers

The Bad

None

The Bottom Line

Would recommend

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