U.S. Biotech Sources, LLC
2% B Agarose Beads Standard
A-1020S-1000
Info: Browse Chromatography and Separation Search Tool
Results of using 2% agarose beads to column purify HPV virus-like particle from cell lysate of 293T cell line. The product is easy to use and economical.
Purify virus-like particle from cell lysate
Cell lysate of 293T cell expressing HPV virus-like particle
1. Column size: 4 mL 2. Load 200 µL cell lysate 3. Collect factions, 0.25 mL/fraction 4. Run SDS gel to check fractions
I changed the agarose beads after about 50 uses.
Virus-like particle size: 23832 kDa, comes from fractions 6–12. Can be separate from RNase A and most of other protein in cell lysate.
N/A
Easy to use, economical
Only good for separate particles having very large molecular weight
Economical and easy method for purifying protein particles with large molecular weight.