PDTC Inhibits HOCl-LDL-Induced CaMKII Oxidation

January 11, 2019

BlueskyReddit
Medical University of Graz
Department of Cardiology
Postdoctoral Researcher

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Company:

Tocris Bioscience

Product Name:

Pyrrolidinedithiocarbamate ammonium

Catalog Number:

0727/50

HL-1 cardiomyocytes were pre-treated with Pyrrolidinedithiocarbamate (PDTC, 1 mM), Tempol (1 mM) or N-Acetylcysteine (NAC, 1 mM) for 30 min and stimulated with HOCl-LDL (200 µg/ml) for 2 hr to follow CaMKII oxidation using Western blot.

Experimental Design and Results Summary

Application

Molecular Biology

Starting Material

Cardiomyocytes

Protocol Overview

HL-1 cardiomyocytes were pre-treated with Pyrrolidinedithiocarbamate (PDTC, 1 mM), Tempol (1 mM) or N-Acetylcysteine (NAC, 1 mM) for 30 min and stimulated with HOCl-LDL (200 µg/ml) for 2 hr to follow CaMKII oxidation using Western blot.

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Results Summary

PDTC blocked HOCl-LDL-induced CaMKII oxidation in cardiomyocytes

DOI or PMID #

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Additional Notes

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Summary

The Good

Works as expected

The Bad

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The Bottom Line

PDTC inhibits HOCl-LDL-induced CaMKII oxidation

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