Perfect Western Blot Results

February 12, 2019

BlueskyReddit
Molecular Biology
Princeton
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Ezh2 (D2C9) XP® Rabbit mAb

Catalog Number:

5246

Highly specific antibody with great signal- detects basal EZH2 expression in cells

Experimental Design and Results Summary

Applications

Western Blot

Sample

MDA-MB-231 derivatives with EZH2 knockdown

Primary Incubation

1:1000

Blocking Agent

5% milk

Secondary Incubation

HRP anti-Rabbit

Tertiary Incubation

none

Detection

Chemiluminescence

Results Summary

Results showed a single, clear band after only a short exposure that was confirmed to be EZH2 by shRNA KD. Excellent antibody

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works perfectly

The Bad

None

The Bottom Line

Best antibody available for EZH2

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