Anti-CD162 Does Not Work By Western Blot

February 27, 2019

BlueskyReddit
Molecular Biology
Princeton
Graduate Student

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Company:

eBioscience

Product Name:

CD162 (PSGL-1) Monoclonal Antibody

Catalog Number:

BMS164

Cells with verified PSGL-1 expression were tested by western. No signal was detected.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse 4T1 cells and human MDA-MB-231

Primary Incubation

1:1000

Blocking Agent

5% milk

Secondary Incubation

Licro IRDye800 CM

Tertiary Incubation

none

Detection

Licor Odyssey

Results Summary

Results showed only one non-specific low molecular weight band. No other bands were detected indicating this antibody doesn't work on mouse or human lysates by western blot.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

None

The Bad

Doesn't work

The Bottom Line

Find a different antibody for PSGL-1

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