Usable Anti-Human CD3 With Some Background Staining

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

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Company:

BD Biosciences

Product Name:

BUV395 Mouse Anti-Human CD3

Catalog Number:

563546

Browse Similar Products: CD3 Flow Cytometry Antibodies

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining B cells (CD19+) and T cells (CD3+) in isolated PBMCs from peripheral blood of a healthy donor (HD).

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Isolated PBMCs from HD peripheral blood

Primary Incubation

anti CD3 BUV395, 15 min, 4 °C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

We identified a clear population of T cells expressing CD3.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

It works

The Bad

background staining and the price

The Bottom Line

It is useful to increase the flow panel.

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