BD Biosciences
Mouse monoclonal anti-NOS2/iNOS antibody
610328
Browse Similar Products: NOS2 Western Blot Antibodies
Quantification of Western blot analysis showed that iNOS increased the activation of the Notch1 signal pathway, and this increase was blocked by the iNOS inhibitors.
Western Blot
Cell lysates
1/1000
5% milk
1/2000
Room temperature 1 hour
ECL
Cells were lysed with the buffer [1% SDS, 10 mmol/L Tris-Cl (pH 7.6), 20 µg/µL aprotinin, 20 µg/µL leupeptin, and 1 mmol/L 4-(2-aminoethyl)benzenosulfonyl fluoride]. The protein concentrations were determined using the Bicinchoninic Acid Protein Assay kit (Pierce, Rockford, IL). Twenty micrograms of protein were separated on SDS-PAGE gels and transferred to polyvinylidene difluoride membranes. After blocking, the membranes were incubated with the appropriate primary antibody at 4°C overnight. After washing with TBST, the membranes were incubated with HRP-conjugated secondary antibodies at room temperature for 1 hour. Proteins were detected with the enhanced chemiluminescence kit (Amersham Pharmacia Biotechnology, Inc., Piscataway, NJ). Quantification of Western blot analysis showed that iNOS increased the activation of the Notch1 signal pathway, and this increase was blocked by the iNOS inhibitors BYK191023 (10 μM) and 1400 W (100 μM) (*P < 0.05, t test).
doi/10.1073/pnas.1722100115
Figure 3F
Worked well
N/A
Worked as expected