Calbiochem (MilliporeSigma)
Human Recombinant TACE
PF133
Info: View TACE Proteins View Product
A fluorometric TACE activity assay kit (SensoLyte 520) with Human Recombinant TACE (Calbiochem, Cat#PF133) was used to measure TACE activation
Quantification of TACE Activity
Cells
Follow the fluorometric TACE activity assay kit (SensoLyte 520) (AS-72085)
TACE activation [expressed as activity in relative fluorescence units (RFU)] was significantly increased in CD24+CD133+ cells after Ad-iNOS transfection compared with Ad-iNOS–transduced CD24−CD133− cells, and this elevation was prevented by inhibition of iNOS (1400 W; 100 μM), or TACE (TAPI-2; 20 μM) for 6 h.
doi/10.1073/pnas.1722100115
Figure 4A
Worked well
N/A
Worked as expected