TACE Activity Assay Kit (SensoLyte 520)

University of Pittsburgh
Surgery
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Anaspec, Inc.

Product Name:

TACE activity assay kit (SensoLyte 520)

Catalog Number:

AS-72085

This TACE activity assay kit was used to measure TACE activation [expressed as activity in relative fluorescence units (RFU)].

Experimental Design and Results Summary

Application

Quantification of TACE Activity

Starting Material

CD24+CD133+ cells

Protocol Overview

TACE activation [expressed as activity in relative fluorescence units (RFU)] was significantly increased in CD24+CD133+ cells after Ad-iNOS transfection compared with Ad-iNOS–transduced CD24−CD133− cells, and this elevation was prevented by inhibition of iNOS (1400 W; 100 μM), or TACE (TAPI-2; 20 μM) for 6 h.

Tips

Follow the fluorometric TACE activity assay kit (SensoLyte 520) (AS-72085)

Results Summary

TACE activation [expressed as activity in relative fluorescence units (RFU)] was significantly increased in CD24+CD133+ cells after Ad-iNOS transfection compared with Ad-iNOS–transduced CD24−CD133− cells, and this elevation was prevented by inhibition of iNOS (1400 W; 100 μM), or TACE (TAPI-2; 20 μM) for 6 h.

DOI or PMID #

doi/10.1073/pnas.1722100115

Additional Notes

Figure 4A

Related Categories

Image Gallery

Summary

The Good

Worked well

The Bad

N/A

The Bottom Line

Worked as expected

Join the discussion