Miltenyi Biotec
Isotype mouse IgG2b
130-092-217
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FACS were performed with Mouse IgG2b isotype control antibody in parallel with CD133-APC to exclude false positive cells. In summary, viable CD133+/CD133- populations were sorted with isotype control -based gating parameters.
Flow Cytometry
Human hepatoma cell carcinoma tissue specimens
30 min at 4°C
N/A
APC
For FACS sorting or analysis of the CD133 epitope, single cells were stained for 30 min at 4°C with monoclonal antibody CD133-APC (CD133/2; Miltenyi Biotec, 10 μl per test up to 10^7 cells in a total volume of 100 μL buffer). Viable CD133+/CD133- populations were sorted with isotype control-based gating parameters. Mouse IgG2b isotype control antibody (356475) was used in parallel with CD133-APC to exclude false positive cells.
doi/10.1073/pnas.1722100115
Figure 1
Worked well
Worked as expected