Human Antibody CD133-APC

February 11, 2019

Surgery
University of Pittsburgh
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

Human antibody CD133-APC

Catalog Number:

130-113-184

For FACS sorting or analysis of the CD133 epitope, single cells were stained for 30 min at 4°C with monoclonal antibody CD133-APC (CD133/2; Miltenyi Biotec, 10 μl per test up to 10^7 cells in a total volume of 100 μL buffer). Viable CD133+/CD133- populations were sorted with isotype control -based gating parameters.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human hepotoma cell carcinoma tissue specimens

Primary Incubation

1/50; 30 min, 4C

Blocking Agent

n/a

Secondary Incubation

n/a

Tertiary Incubation

n/a

Detection

APC

Results Summary

For FACS sorting or analysis of the CD133 epitope, single cells were stained for 30 min at 4°C with monoclonal antibody CD133-APC (CD133/2; Miltenyi Biotec, 10 μl per test up to 10^7 cells in a total volume of 100 μL buffer). Viable CD133+/CD133- populations were sorted with isotype control -based gating parameters.

DOI or PMID #

doi/10.1073/pnas.1722100115

Additional Notes

Figure 1

Related Categories

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Summary

The Good

Worked well

The Bad

n/a

The Bottom Line

Worked as expected

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