SAHA Inhibitor

University of Pittsburgh
Surgery
Research Scientist

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Company:

Cayman Chemicals

Product Name:

SAHA

Catalog Number:

CAS 149647-78-9

UPCI15B and UMSCC-1 cells were treated with SAHA and 5-aza-dC alone or in combination for 48 h. Cells were harvested for mRNA extraction and Western blotting. Levels of PKD1 transcripts were determined by real time qRT-PCR. Protein expression was analyzed by immunoblotting for PKD1.

Experimental Design and Results Summary

Application

Cell treatment

Starting Material

UPCI15B and UMSCC-1 cells

Protocol Overview

UPCI15B and UMSCC-1 cells were treated with SAHA and 5-aza-dC alone or in combination for 48 h. Cells were harvested for mRNA extraction and Western blotting. Levels of PKD1 transcripts were determined by real time qRT-PCR. Protein expression was analyzed by immunoblotting for PKD1.

Tips

Solubility of the reagents

Results Summary

Levels of PKD1 transcripts were determined by real time qRT-PCR. Protein expression was analyzed by immunoblotting for PKD1.

DOI or PMID #

https://doi.org/10.1186/s12885-018-4965-6

Additional Notes

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Summary

The Good

Worked well

The Bad

N/A

The Bottom Line

Worked as expected

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