Time Course Effect Of Chemoattractant On ASK1 Phosphorylation

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Cell Signaling

Product Name:

Phospho-ASK1 (Thr845) Antibody

Catalog Number:

3765

ASK1 is a MAP kinase, plays crucial role in stress-induced apoptosis. ASK1 is activated in response to a variety of stress-related stimuli through distinct mechanisms and activates MKK4 and MKK3, which in turn activate JNK and p38. Overexpression of ASK1 activates JNK and p38 and induces apoptosis in several cell types through signals involving the mitochondrial cell death pathway. Here checked the time course effect of ASK1 phosphorylation by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lysate

Primary Incubation

1:1000 dilution prepared in 1x TBST

Blocking Agent

5% BSA prepared in 1x TBST

Secondary Incubation

1:4000 dilution prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

There is a significant increase in ASK1 phosphorylstion by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

This antibody is very good for western blot experiment

The Bad

None

The Bottom Line

This antibody works great for western blot. Signal is good and very specific. Highly recommended.

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