DPP9/DPRP2 Antibody For Western Blot

February 12, 2019

Department of Cardiology
Medical University of Graz
Postdoctoral Researcher

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Company:

Santa Cruz Biotechnology, Inc.

Product Name:

DPRP2 (F-1) Antibody

Catalog Number:

sc-271634

This antibody was used for detection of DPP9 in cardiomyocytes. Antibody detected DPP9 at 98 kDa in HL-1 mouse atrial cardiomyocyte cell line.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HL-1 cells

Primary Incubation

Dilution 1:500 in 5% BSA-TBST, incubation at 4°C for 16 h

Blocking Agent

5% non-fat milk in TBST

Secondary Incubation

HRP-conjugated anti-mouse antibody (1:1000, Cell Signaling) at 25°C for 2 h

Tertiary Incubation

N/A

Detection

Immobilon Western HRP Substrat

Results Summary

This antibody detected DPP9 immunoreactive band at 98 kDa in protein lysates of HL-1 mouse cardiomyocytes and mouse left ventricle (LV).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Very specific antibody

The Bad

N/A

The Bottom Line

This antibody can be used for DPP9 detection using Western blot.

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