Excellent Anti-CD45 Antibody, No Optimization Needed

January 25, 2019

BlueskyReddit
Molecular Biology
Princeton
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

CD45 Monoclonal Antibody (30-F11), APC

Catalog Number:

17-0451-82

Used on mouse immune populations, excellent signal above background and bright staining from APC. In summary, clearly differentiated CD45+ immune cells from remaining stromal cells in the spleen, bone marrow, etc.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1:100 in PBS

Blocking Agent

none

Secondary Incubation

none

Tertiary Incubation

none

Detection

APC laser LSR2

Results Summary

Clearly differentiated CD45+ immune cells from remaining stromal cells in the spleen, bone marrow, etc.Used at 1:100 is cost-effective and provides very strong signal.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Excellent staining for good price

The Bad

nothing

The Bottom Line

One-stop shop for CD45 antibody

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