Low Activity Recombinant Protein, Not Much Use At Supplied Amount

Princeton
Molecular Biology
Graduate Student

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Company:

Novoprotein

Product Name:

Recombinant human Aldh1a2

Catalog Number:

CP08

This was used for a NAD reduction assay to measure activity or various inhibitors shown to inhibit mammalian 1a2. This worked very poorly with low activity in the biochemical assay.

Experimental Design and Results Summary

Application

Enzymatic assay

Starting Material

N/A

Protocol Overview

Product was used at 1:100 with NAD+, MTT and propanal to assess reduction of NAD and corresponding oxidation of propanal. Activity was very low even at 2 hours.

Tips

Use at a higher concentration than 1:100

Results Summary

Results showed that while this product could oxidize propanal, it performed much more slowly than other reagents or the published literature indicate.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Specific activity toward propanal

The Bad

Very low activity at a high cost

The Bottom Line

Consider your own recombinant expression before using this product, not worth the convenience of ordering.

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