Cell Signaling Technology
Phospho-Akt (Thr308) (D25E6) XP® Rabbit mAb
#13038
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Insulin receptor signaling leads to the phosphorylation of Akt at 2 residues- ser 473 and thr308, which can be detected by western blotting.
Western Blot
Murine T Cell lysates
1/1000 4*C overnight in 5% BSA
5% BSA in TBS
1/1000 anti rabbit HRP, 1 hour room temp
Not required
Chemiluminescence
Works well in detecting Akt phosphorylation at Thr308
N/A
Works as expected
Would recommend for the detection of pThr 308 on Akt