Anti-Mouse PE IL6Ra Antibody

January 29, 2019

Advanced Diagnostics
University Health Network
Postdoctoral Fellow

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Company:

BioLegend

Product Name:

PE anti-mouse CD126 (IL-6Rα chain) Antibody

Catalog Number:

115805

We used this antibody for identification of IL-6Ra expression in Neutrophil subsets. The antibody specifically labels IL6Ra but is not very bright.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

15 minutes

Blocking Agent

Anti-CD16/CD32 antibody

Secondary Incubation

30 minutes

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

Surface expression of IL6Ra on splenic neutrophils

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

It specifically labels IL6Ra.

The Bad

It does not provide a very bright signal.

The Bottom Line

PE IL6Ra antibody is useful to label IL6Ra expression. However, it does not give a very bright signal.

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