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Anti-human CD25 FITC
356106
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Human peripheral T cells were stimulated with staphylococcus enterotoxin B and activation read out by CD25 staining. The antibody was bright and had a dynamic signal.
Flow Cytometry
Human PBMC
1 µL/test, RT, 15 min
Housemade blocking buffer containing normal sera
N/A
LSR Fortessa
Primary human PBMC were stimulated with SEB for 3 days and T cell activation was determined by CD25 staining. The antibody does give a very bright and dynamic signal. (Blue histogram=unstim, Orange histogram=TLR7 agonist.) The antibody can be used at 1/5 of the recommended test size (1 µL/test instead of 5 µL/test) and still delivers a very dynamic signal.
Very bright, dynamic signal
No complaints
Excellent antibody to read out T cell activation